No. However, there are a few caveats. If you never setup a thorough safelist, some of your processes may be shut off.  You will need to restart your computer to return normal operating conditions. If you have setup your "safelist," you do not have to restart your computer.

Scrubit must be run to completion. This requires a restart. If you choose to abort a testing process, your system may only be partially operational. For this reason, certain safeguards are in place to minimize inconvenience. The "Stop" button resets Scrubit and a safelist protects your safe running processes from testing so you can avoid a restart. If you are unsure and just want to play it safe, simply restart your computer.